Composite

Part:BBa_K203104:Design

Designed by: Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)


mCherry-Myrpalm; PM targetted Red fluorescent protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 86
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 820
    Illegal AgeI site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 46


Design Notes

Mutagenesis PCR to remove BBb site from mCherry protein.


Source

PCR amplified from Plasmid DNA.

References